Inhibitor proteins of a protease and use thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/09 (2006.01) C12N 9/64 (2006.01) C12N 15/15 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2521410

The present invention relates to a chimeric inhibitor protein of a protease comprising an inhibiting polypeptidic sequence and at least one polypeptidic sequence of a substrate-enzyme interaction site specific for a protease. Other objects of the invention are to provide a purified and isolated DNA sequence encoding the chimeric inhibitor protein of a protease, an expression vector characterized in that it comprises said purified and isolated DNA sequence, a eukaryotic or prokaryotic host cell transformed with this expression vector and a method of producing a chimeric inhibitor protein.

La prEsente invention concerne une protEine chimErique d'une protEase, comportant une sEquence polypeptidique inhibitrice et au moins une sEquence polypeptidique d'un site d'interaction substrat-enzyme spEcifique contre une protEase. L'invention concerne d'autre part une sEquence d'ADN purifiEe et isolEe codant pour la protEine inhibitrice chimErique d'une protEase, un vecteur d'expression caractErisE en ce qu'il renferme ladite sEquence d'ADN, une cellule hOte eucaryote ou procaryote transformEe avec ce vecteur d'expression, et un procEdE pour produire une protEine inhibitrice chimErique.

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