Initial rate photometric method for immunoassay

Details Initial rate photometric method for immunoassay Initial rate photometric method for immunoassay

G - Physics

01

N

G01N 33/557 (2006.01) G01N 21/27 (2006.01)

Patent

CA 2116687

2116687 9402853 PCTABS00030 An initial rate photometric immunoassay method useful for detecting and quantifying analytes in various physiological fluids is disclosed. The method is carried out by combining in a liquid medium an undiluted sample of analyte-containing physiological fluid, such as serum, and an excess of an anti-analyte antibody. A substantially constant initial rate of increase of the liquid medium'sturbidity, due to the resulting immunoprecipitation reaction, is measured in real time and compared to a calibration curve prepared from known analyte concentrations to detect and quantify the analyte. Detection and quantification of numerous analytes including haptens, drugs and proteins, in a wide variety of physiological fluids can be rapidly carried out by the present method.

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