Injecting drosophila embryos

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/89 (2006.01) C12N 15/00 (2006.01) C12N 15/10 (2006.01) C12N 15/87 (2006.01) C12Q 1/68 (2006.01) C40B 50/06 (2006.01)

Patent

CA 2706100

The present invention provides systems that allow reliable multiplexed transformation of Drosophila embryos. The present invention provides methods and reagents that allow preparation of injection-quality nucleic acid samples and that allow simultaneous preparation of multiple such samples. The present invention provides systems for simultaneous processing of multiple injected embryos. The present invention provides methods for transformation of Drosophila embryos involving use of virginator strains that can be used to increase the efficiency of setting up the crosses needed to produce the eggs for the injections and for the crosses needed to screen for transformants.

L'invention concerne des systèmes qui permettent la transformation multiplexée fiable d'embryons de Drosophila. La présente invention propose des procédés et des réactifs qui permettent la préparation d'échantillons d'acide nucléique de qualité d'injection et qui permettent la préparation simultanée de multiples tels échantillons. La présente invention propose des systèmes pour le traitement simultané de multiples embryons injectés. La présente invention propose des procédés de transformation d'embryons de Drosophila impliquant l'utilisation de souches de virginateur qui peuvent être utilisées pour accroître l'efficacité de l'établissement des croisements nécessaires pour produire les ufs pour les injections et pour les croisements nécessaires pour cribler des transformants.

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