C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/19 (2006.01) C12N 15/52 (2006.01) C12N 15/81 (2006.01) C12P 7/02 (2006.01) C12P 7/18 (2006.01)
Patent
CA 2063195
A yeast cell, preferably Saccharomyces cerevisiae, which contains a functional stable recombinant DNA sequence that does not allow for the encoding of a negative regulator of phospholipid biosynthesis therein and which has multiple copies of an INO1 gene. In a preferred embodiment, the recombinant DNA sequence is an OPI1 gene deletion which results in the deregulation of inositol or inositol-containing metabolites such as inositol-1-phosphate synthase. Moreover, there is a method for obtaining inositol, inositol-containing metabolites or phospholipids such as myo-inositol or inositol-1-phosphate. The method comprises the steps of genetically engineering a stable yeast cell, preferably Saccharomyces cerevisiae, to continually produce inositol, inositol-containing metabolites or phospholipids. Additionally, them is the step of then generating the inositol, inositol-containing metabolites or phospholipids. In a preferred embodiment, the genetically engineering step includes the step of altering the negative regulatory step involved in phospholipid biosynthesis to overproduce inositol, inositol-containing metabolites or phospholipids such as myo-inositol.
Henry Susan A.
White Michael J.
Borden Ladner Gervais Llp
Carnegie-Mellon University
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