Insect sequences for improving the efficiency of secretion...

Details Insect sequences for improving the efficiency of secretion... Insect sequences for improving the efficiency of secretion...

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 14/435 (2006.01) C12N 5/10 (2006.01) C12N 9/16 (2006.01) C12N 9/18 (2006.01) C12N 15/55 (2006.01) C12N 15/62 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2301334

An expression cassette useful for the secretion of a heterologous protein from eukaryotic cells comprising a DNA sequence encoding a promoter functionally linked to a DNA sequence coding for the juvenile hormone esterase gene which is linked in frame to a heterologous gene is disclosed. Also disclosed is a method of secreting heterologous proteins in eukaryotic cells.

Cette invention concerne une cassette d'expression qui est utile pour la sécrétion d'une protéine hétérologue par des cellules eucaryotes. Cette cassette comprend une séquence d'ADN codant un promoteur qui est lié fonctionnellement à une séquence d'ADN codant pour le gène d'estérase d'hormone juvénile, ce gène étant lui-même lié en phase à un gène hétérologue. Cette invention concerne également un procédé de sécrétion de protéines hétérologues dans des cellules eucaryotes.

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