Insecticidal factor from field peas

A - Human Necessities – 01 – N

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A01N 65/20 (2009.01) A01P 7/04 (2006.01)

Patent

CA 2278501

A method and composition is provided to control insect pests using pea extracts. Pea extracts, protein-rich, fibre-rich and starch-rich fractions were tested for toxicity against various grain storage pests. The pea protein- rich extract was found to be toxic against the lesser grain borer, red flour beetle, rice weevil, maize weevil, granary weevil and the rusty grain beetle. The pea protein-rich extract also reduced the number of offspring of all insects tested. The protein-rich fraction was also found to be effective against the bertha army worm, diamondback, grasshopper, Indian meal moth and flea beetle. A partially purified active extract from the protein-rich fraction has been identified, using ion-exchange chromatography, reversed- phased chromatography, adsorption chromatography and gel filtration. However, the active ingredient within this fraction is not a protein greater than about 4000 daltons, nor is it a protease inhibitor, lectin or soyasaponin I.

L'invention concerne un procédé et une composition permettant de lutter contre les insectes nuisibles au moyen d'extraits de pois. Des extraits de pois, des fractions riches en protéines, riches en fibres et riches en amidon ont été testés afin de déterminer leur toxicité envers divers nuisibles des installations de stockage des céréales. L'extrait de pois riche en protéines s'est avéré toxique pour le petit perceur de céréales, le tribolium rouge de la farine, le charançon du riz, le charançon du maïs, le calandre des grains et le cucujide roux. Il a réduit la prolifération de tous les insectes testés. Il s'est également avéré efficace contre le légionnaire bertha, la teigne des crucifères, la sauterelle, la pyrale indienne de la farine et la puce terrestre. Un extrait actif partiellement purifié obtenu à partir de la fraction riche en protéines a été identifié par chromatographie par échanges d'ions, chromatographie à phase inversée, chromatographie d'adsorption et filtration sur gel. Mais le principe actif contenu dans cette fraction n'est ni une protéine de plus de 4000 daltons, ni un inhibiteur des protéases, ni une lectine, ni une saponine I du soja.

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