C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/32 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) A01N 63/02 (2006.01) A01P 7/04 (2006.01) C07K 14/325 (2006.01) C12N 1/21 (2006.01) C12N 5/04 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01) C12P 19/34 (2006.01) C12P 21/02 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2531400
The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against coleopteran insects including Colorado potato beetle (Lymantria dispar) and Southern Corn Rootworm (Diabrotica undecempunctata). Insecticidal proteins encoded by nucleotide sequences that hybridize to the isolated and characterized nucleotide sequences are disclosed. Also disclosed are methods of making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.
La présente invention concerne l'isolement et la caractérisation de séquences nucléotidiques codant pour de nouvelles protéines insecticides sécrétées dans l'espace extracellulaire de Bacillus thuringiensis et des souches associées. Les protéines sont isolées de surnageants de culture de Bacillus thuringiensis et des souches associées, et présentent une activité insecticide contre les insectes coléoptères tels que le doryphore de la pomme de terre (Lymantria dispar) et la chrysomèle occidentale des racines du maïs (Diabrotica undecempunctata). L'invention concerne également des protéines insecticides codées par des séquences nucléotidiques s'hybridant avec les séquences nucléotidiques isolées et caractérisées. L'invention concerne également des méthodes de fabrication et d'utilisation de cellules et de plantes transgéniques contenant la nouvelle séquence nucléotidique de l'invention.
Baum James A.
Donovan Judith C.
Donovan William P.
Engleman James T.
Krasomil-Osterfeld Karina
Mcfadden Fincham
Monsanto Technology Llc
LandOfFree
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