Insertional mutagenesis technique

Details Insertional mutagenesis technique Insertional mutagenesis technique

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07K 14/435 (2006.01) C12N 9/22 (2006.01) C12N 15/52 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2437309

The invention provides a method for producing a library of genetic mutations in a cell population by insertional mutagenesis, wherein a viral vector comprising a transposon is used to deliver said transposon to said cell population, which cell population stably expresses the cognate transposase for said transposon, and the transposon is mobilised to give rise to the genetic mutations.

L'invention concerne une méthode de production d'une bibliothèque de mutations génétiques dans une population cellulaire par mutagenèse insertionnelle, dans laquelle un vecteur viral portant un transposon est utilisé pour fournir ledit transposon à ladite population cellulaire. Cette population cellulaire exprime de façon stable le transposase correspondant pour ledit transposon, le transposon étant mobilisé pour entraîner les mutations génétiques visées.

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