Insertional mutations in mycobacteria

Details Insertional mutations in mycobacteria Insertional mutations in mycobacteria

C - Chemistry, Metallurgy

12

N

C12N 1/21 (2006.01) A61K 35/66 (2006.01) A61K 39/04 (2006.01) A61K 39/116 (2006.01) A61K 39/295 (2006.01) C07K 14/35 (2006.01) C12N 15/74 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2111217

2111217 9222326 PCTABS00018 A mutated mycobacterium selected from the class consisting of mutated M.bovis-BCG, mutated M.tuberculosis, and mutated M.leprae. The mutation of M.bovis-BCG, M.tuberculosis, or M.leprae is preferably effected through an insertional mutation of a mycobacterial gene. The insertional mutagenesis may be effected, for example, through illegitimate recombination or by a mycobacterial transposon. Such mutated mycobacteria may then be transformed with an expression vector(s) containing a complement gene to the gene which is mutated, and preferably also including a heterologous gene.

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