Insolubilized glucose isomerase

C - Chemistry – Metallurgy – 12 – P

Patent

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195/139, 195/35.

C12P 19/00 (2006.01)

Patent

CA 1045999

ABSTRACT OF THE DISCLOSURE The present invention discloses an insolubilized glucose isomerase consisting of glucose isomerase which is adsorbed and bound on a porous type anion exchange resin having a porosity of more than 4.5% (measured by aqueous dextran solution method) and an ion exchange capacity of more than 0.035 meq/ml-Resin (measure by polyanion salt decomposition method). The resin matrix is a styrene-divinyl benzene copolymer, the anion exchange group of the resin being a quarternary ammonium group. The advantages of this invention include (a) more isomerase is adsorbed on the ion exchange resin, (b) higher enzyme activity of the product in the operation, (c) higher resistance to crush and distortion, and (d) less pressure loss as the glucose solution passes through the column containing the insolubilized isomerase.

224106

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