Installation of genomes or partial genomes into cells or...

C - Chemistry – Metallurgy – 12 – P

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Details

C12P 19/34 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C12N 1/00 (2006.01) C12N 1/20 (2006.01) C12N 5/02 (2006.01) C12N 5/04 (2006.01) C12N 15/64 (2006.01)

Patent

CA 2643136

A method is provided for introducing a genome into a cell or cell-like system. The introduced genome may occur in nature, be manmade with or without automation, or may be a hybrid of naturally occurring and manmade materials. The genome is obtained outside of a cell with minimal damage. Materials such as a proteins, RNAs, polycations, nucleoid condensation proteins, or gene translation systems may accompany the genome. The genome is installed into a naturally occurring cell or into a manmade cell-like system. A cell-like system or synthetic cell resulting from the practice of the provided method may be designed and used to yield gene- expression products, such as desired proteins. By enabling the synthesis of cells or cell-like systems comprising a wide variety of genomes, accompanying materials and membrane types, the provided method makes possible a broader field of experimentation and bioengineering than has been available using prior art methods.

L'invention concerne l'introduction d'un génome dans une cellule ou un système de type cellule. Le génome introduit peut être naturel, synthétique avec ou sans automation, ou peut être un hybride de matériaux naturels et synthétiques. Le génome est sorti d'une cellule avec des dommages minimaux. Des matériaux tels que des protéines, des ARN, des polycations, des protéines de condensation de nucléotides ou des systèmes de traduction de gènes peuvent accompagner le génome. Le génome est installé dans une cellule naturelle ou dans un système synthétique de type cellule. Un système de type cellule ou une cellule synthétique résultant de la mise en AEuvre du procédé selon l'invention peut être conçu et utilisé pour obtenir des produits d'expression génique, tels que des protéines souhaitées. En permettant la synthèse de cellules ou de systèmes de type cellules comprenant une large variété de génomes, matériaux d'accompagnement et types de membranes, le procédé selon l'invention rend possible l'élargissement du domaine d'expérimentation et de biotechnologie disponible en utilisant les procédés de l'art antérieur.

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