Instrument, method and kit for detecting lactobacillus...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12M 1/34 (2006.01) C12N 15/09 (2006.01)

Patent

CA 2559952

It is intended to provide an instrument for detecting bacteria by which bacteria contaminating a test sample can be quickly and exactly determined by a convenient procedure, a method of detecting bacteria and a kit for detecting bacteria. Namely, a microarray type instrument wherein an oligonucleotide based on a base sequence specific to the genus or species, to which the target bacteria belong, is immobilized on the surface of a substrate. The target bacteria in the test sample can be conveniently, quickly and exactly detected and identified depending on the occurrence or non-occurrence of the hybridization between a probe prepared from the test sample and the oligonucleotide immobilized on the surface of the substrate as described above.

L'invention se rapporte à un instrument servant A dEtecter des bactEries, au moyen duquel on peut dEterminer rapidement et avec exactitude, par une procEdure pratique, la prEsence de bactEries contaminant un Echantillon testE, à un procEdE de dEtection de bactEries et à un kit servant A dEtecter des bactEries. L'invention concerne notamment un instrument de type puce A ADN dans lequel est immobilisE sur la surface d'un substrat un oligonuclEotide basE sur une sEquence de bases spEcifique du genre ou de l'espEce auquel les bactEries cibles appartiennent. Les bactEries cibles prEsentes dans l'Echantillon testE peuvent Etre commodEment, rapidement et avec exactitude dEtectEes et identifiEes selon la survenue ou non de l'hybridation entre une sonde prEparEe A partir de l'Echantillon testE et l'oligonuclEotide immobilisE sur la surface du substrat comme dEcrit ci-dessus.

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