Insulin producing cells derived from human embryonic stem cells

Details Insulin producing cells derived from human embryonic stem cells Insulin producing cells derived from human embryonic stem cells

C - Chemistry, Metallurgy

12

N

C12N 5/0735 (2010.01) C12N 5/071 (2010.01) A61K 35/12 (2006.01) A61K 35/14 (2006.01) A61K 35/39 (2006.01) C12N 5/00 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2447015

The present invention discloses insulin producing cells derived from human embryonic stem cells. Methods of generating, enriching, selecting, cloning and isolating populations of insulin-producing embryonic cell lines are disclosed. insulin production in these cells can be glucose responsive, or otherwise regulatable. Advantageously, these insulin-producing cells can be stable cell lines, derived from stable transfectants of hESC. Methods of using these cells, cell populations or cell lines particularly for cell therapy are disclosed.,

L'invention concerne des cellules produisant de l'insuline, dérivées de cellules souches embryonnaires humaines. L'invention concerne également des procédés permettant de produire, enrichir, sélectionner, cloner et isoler des populations de lignées de cellules embryonnaires produisant de l'insuline. La production d'insuline dans ces cellules peut être sensible au glucose ou peut être régulée d'une autre manière. De façon avantageuse, ces cellules produisant de l'insuline peuvent être des lignées cellulaires stables, dérivées de transfectants stables de hESC. L'invention concerne enfin des procédés d'utilisation de ces cellules, populations cellulaires ou lignées cellulaires, plus spécifiquement en thérapie cellulaire.

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