Intact gene and method of excising and cloning same

C - Chemistry – Metallurgy – 12 – N

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195/1.235

C12N 15/00 (2006.01) C07K 14/445 (2006.01) C12N 15/10 (2006.01)

Patent

CA 1247540

-21- INTACT GENE AND METHOD OF EXCISING AND CLONING SAME Abstract The present invention discloses isolated functionally intact whole genes and method of obtaining the same. The method includes treating genomic DNA with a nuclease and a denaturing agent under controlled conditions. The invention also discloses cloning of said intact whole genes and a library of such cloned genes or any recombinations thereof. The invention is useful in deriving gene products such as m-RNA, S-RNA, t-RNA, polypeptides and the like.

486049

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