C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/85 (2006.01) A61K 48/00 (2006.01) C12N 15/87 (2006.01)
Patent
CA 2463631
A composition comprising recombinant, intact minicells that contain a therapeutic nucleic acid molecule is disclosed. Methods for purifying a preparation of such minicells also are disclosed. Additionally, a genetic transformation method is disclosed, which comprises (i) making recombinant, intact minicells available that contain a plasmid comprised of a first nucleic acid segment, and (ii) bringing the minicells into contact with mammalian cells that are engulfing-competent, such that the minicells are engulfed by the mammalian cells, which thereafter produce an expression product of the first nucleic acid segment.
L'invention concerne une composition comprenant des mini-cellules entières de recombinaison qui contiennent une molécule d'acide nucléique thérapeutique. L'invention concerne également des méthodes permettant de purifier une préparation contenant ces mini-cellules. En outre, l'invention concerne une méthode de transformation génétique, consistant (i) à isoler des mini-cellules entières de recombinaison contenant un plasmide constitué d'un premier segment d'acide nucléique, et (ii) à mettre les mini-cellules en contact avec des cellules mammifères capables d'ingestion, de telle sorte que les mini-cellules soient ingérées par les cellules mammifères, ce qui permet d'obtenir un produit d'expression du premier segment d'acide nucléique.
Brahmbhatt Himanshu
Macdiarmid Jennifer
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Engeneic Gene Therapy Pty Limited
Engeneic Molecular Delivery Pty Ltd
LandOfFree
Intact minicells as vectors for dna transfer and gene... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Intact minicells as vectors for dna transfer and gene..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Intact minicells as vectors for dna transfer and gene... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1792681