Integrated method for the determination of anti-...

G - Physics – 01 – N

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G01N 33/68 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1179258

ABSTRACT OF THE DISCLOSURE: A method is disclosed for determining the anti- streptolysin antibody rating in blood or serum samples, comprising the steps of contacting a solution of known con- centration of oxidized 0-streptolysin with a diluted blood or serum sample, allowing the mixture thus obtained to stand at a temperature of from 15°C to 75°C, adding a solution of a reducing agent, waiting the red blood cell sedimentation, checking if haemolysis occurred and checking the condition of a control blank. If a serum sample is used, it is necessary to add red blood cells thereto prior to the treat- ment with the reducing agent. It is possible to stop the test to the determination of the antibody threshold and to prosecute testing if the preselected threshold has been exceeded. A diagnostic kit containing the reagents necessary for the tests is also provided.

389715

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