Integrated microfluidic sperm isolation and insemination device

Details Integrated microfluidic sperm isolation and insemination device Integrated microfluidic sperm isolation and insemination device

A - Human Necessities

61

D

A61D 19/02 (2006.01) A61B 17/43 (2006.01) C12N 5/076 (2010.01) C12M 3/00 (2006.01)

Patent

CA 2527733

An integrated microfluidic sperm isolation and oocyte insemination device provides the opportunity to perform in vitro insemination with motilityenhanced sperm samples and with minimal manipulation of fragile oocytes. Sperm sorting is performed in a common sort channel wherein more mobile sperm swim across the interface between co-laminar flows of senen and media fluid.

La présente invention concerne un dispositif microfluidique intégré d'isolation de sperme et d'insémination d'ovocytes. Ce dispositif donne la possibilité de réaliser en éprouvettes, avec un minimum de manipulations, l'insémination d'ovocytes fragiles avec des échantillons de sperme à motilité accrue. Le tri du sperme se fait dans un canal commun de tri dans lequel le sperme plus mobile traverse en nageant l'interface entre flux co-laminaires de sperme et de milieu fluidique.

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