Integrated separation of organic substances from an aqueous...

B - Operations – Transporting – 01 – D

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B01D 11/04 (2006.01) C12P 13/04 (2006.01)

Patent

CA 2484863

The invention relates to a method for the integrated separation of one or more organic substances present in an aqueous bio-process mixture containing at least one positively charged and/or chargeable group containing nitrogen by means of reactive extraction in at least one step. According to the invention, at least one liquid-liquid centrifuge is used for said reactive extraction and the organic substances from the extraction agent are re-extracted into an aqueous phase. In particular, cells and protein are removed from the bio- process mixture that is fed to the liquid-liquid centrifuges before said mixture is fed to the first centrifuge. The invention also relates to a method of this type, whereby an aqueous bio-process mixture is continuously removed from a bio-reactor, fed, together with an extraction agent, to a liquid-liquid centrifuge, extracted by means of the extraction agent, thus obtaining an organic phase, which contains the substance to be extracted. The substance can be re-extracted in a circuit using a second liquid-liquid centrifuge, producing a concentrated aqueous solution from the extracted substance and the organic phase is returned to the first liquid-liquid centrifuge.

L'invention concerne un procédé pour la séparation intégrée, par extraction réactive en au moins une étape, d'une ou de plusieurs substances organiques présentes dans un mélange de bioprocédé aqueux et contenant au moins un groupe azoté chargé ou pouvant être chargé positivement. On utilise pour l'extraction réactive au moins une centrifugeuse liquide-liquide et on réextrait les substances organiques de l'agent d'extraction dans une phase aqueuse. On débarrasse notamment le mélange de bioprocédé amené aux centrifugeuses liquide-liquide de ses cellules et protéines avant qu'il ne soit introduit dans la première centrifugeuse. L'invention concerne également un tel procédé selon lequel un mélange aqueux de bioprocédé est extrait en continu d'un bioréacteur, est amené accompagné d'un agent d'extraction dans une centrifugeuse liquide-liquide, est extrait à l'aide de l'agent d'extraction, une phase organique contenant la substance à extraire du mélange de fermentation étant ainsi obtenue. La substance peut être réextraite cycliquement par l'intermédiaire d'une deuxième centrifugeuse liquide-liquide, une solution aqueuse concentrée étant alors produite à partir de la substance extraite, et la phase organique est ramenée dans la première centrifugeuse liquide-liquide.

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