B - Operations – Transporting – 01 – D
Patent
B - Operations, Transporting
01
D
204/171.7
B01D 57/02 (2006.01) G01N 27/447 (2006.01) G01N 27/453 (2006.01) G01N 21/03 (2006.01)
Patent
CA 2024092
ABSTRACT OF THE DISCLOSURE An integrated temperature control/alignment system for use with a high performance capillary electrophoretic apparatus comprises a complementary pair of capillary column mounting plates formed from an electrically insulative, high thermal conductivity material for mounting a capillary column. The first mounting plate includes a well having a predetermined circumference, first and second grooves contiguous with the well and ports formed in the grooves and extending through the plate. The second mounting plate includes a plug configured for insertion in the cell and ports aligned with the ports of the first mounting plate. Respective ends of the capillary column are seated and locked in the grooves to preposition the capillary column with respect to the ports. Capillary columns of varying lengths may be mounted within the mounting plates by coiling the intermediate portion thereof within the well to increase or decrease the working length of the column in predetermined increments, depending upon the particular application. Optical coupling elements are disposed in the ports to couple sampling and reference radiation to and from the mounted capillary column. The temperature of substantially the entire capillary column is controlled or regulated by at least one thermopile disposed in thermal contact with one of the mounting plates. A thermistor may be thermally coupled to the capillary column or one of the mounting plates to provide feedback signals to operate the thermopile to maintain the capillary column at a predetermined electrophoretic operating temperature.
Karger Barry L.
Nelson Robert J.
Malcolm Johnston & Associates
Northeastern University
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