Integration cassette for improvement of transgenesis in...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/90 (2006.01) C12N 15/64 (2006.01) C12N 15/79 (2006.01)

Patent

CA 2257096

The present invention relates to DNA constructs provided to improve transgenesis or genomic integration of DNA fragment of interest into nuclear DNA of eukaryotic cell. The DNA constructs of the present invention allow a more efficient procedure by increasing the rate of genomic integration of a DNA fragment. After integration into host cell's DNA, the DNA constructs may provide a mean of screening transgenic cells. An increasing integration means, as well as a method for screening transgenic cells with said DNA construct are disclosed.

La présente invention concerne des produits de recombinaison d'ADN destinés à améliorer la transgénèse ou l'intégration génomique d'un fragment d'ADN déterminé dans l'ADN nucléaire d'une cellule eucaryote. Les produits de recombinaison d'ADN de la présente invention rendent la procédure plus efficace en accélérant la vitesse d'intégration génomique d'un fragment d'ADN. Une fois intégrés dans l'ADN de la cellule hôte, les produits de recombinaison d'ADN peuvent constituer un moyen de criblage des cellules transgéniques. On décrit également un moyen d'intégration plus grande ainsi qu'un procédé permettant de cribler les cellules transgéniques à l'aide desdits produits de recombinaison d'ADN.

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