Integrative gene-expression in food-grade microorganisms

Details Integrative gene-expression in food-grade microorganisms Integrative gene-expression in food-grade microorganisms

C - Chemistry, Metallurgy

12

N

C12N 15/87 (2006.01) C12N 9/10 (2006.01) C12N 15/63 (2006.01) C12N 15/74 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2093152

A process for integrating a promoter-less foreign gene into an operon in front of at least one essential cistron on the DNA of a food-grade microorganism, in such a way that the gene is expressed as functional part of this operon and that the gene is stably maintained and expressed due to selective pressure on correct functioning of the essential cistron upon growth in standard medium, the integration being preferably carried out by transforming said microorganism with a donor plasmid which is not able to replicate by itself in the microorganism and which carries said foreign gene as a functional part of said operon in front of said essential cistron.

Processus pour l'intégration d'un gène étranger en l'absence de promoteur dans un opéron devant au moins un cistron essentiel de l'ADN d'un micro-organisme de qualité alimentaire, de manière que le gène soit exprimé en tant que partie fonctionnelle de cet opéron et que le gène soit maintenu de façon stable et exprimé par suite de la pression sélective exercée sur le fonctionnement correct du cistron après croissance dans un milieu standard, l'intégration étant de préférence effectuée par la transformation dudit microorganisme au moyen d'un plasmide donneur qui est incapable de se reproduire par lui-même dans le micro-organisme et qui transporte ledit gène étranger en tant que partie fonctionnelle dudit opéron devant ledit cistron essentiel.

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