Interaction of colloid-immobilized species with species on...

G - Physics – 01 – N

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G01N 33/543 (2006.01)

Patent

CA 2360927

The invention provides novel techniques for derivatizing colloids with self- assembled monolayers. This provides the capability of a wide variety of assays including chemical or biochemical agent/agent interaction studies. Bio- derivatized colloids, with or without signaling entities, are used to probe interactions with species on non-colloidal structures. The invention provides techniques for immobilizing colloidal particles on a wide variety of non- colloidal structures. Included is the ability to decorate a variety of non- colloidal structures including beads, with colloids as a detectable assay. This allows, in many cases, assays detectable via the unaided human eye, as well as assays detectable via automated determination of a change of interaction of electromagnetic radiation with the colloids, e.g., absorption, light-scattering, and the like.

La présente invention concerne de nouveaux procédés permettant de dériver des colloïdes avec des monocouches auto-assemblées. Cela offre la possibilité d'une grande diversité d'essais et notamment des études à interaction entre agents chimiques ou biochimiques. De tels colloïdes dérivés biologiquement, avec ou sans entités de signalisation, servent comme sonde recherchant des interactions avec des espèces existant sur des structures non colloïdales. L'invention comporte ainsi des techniques permettant d'immobiliser des particules colloïdales sur une grande diversité de structures non colloïdales. On a même la possibilité de munir de colloïdes diverses structures non colloïdales, y-compris des billes, ce qui permet de réaliser un essai détectable. Cela permet, dans de nombreux cas, des essais détectables à l'oeil nu, ainsi que des essais détectables par détermination automatisée d'une modification de l'interaction d'un rayonnement électromagnétique avec les colloïdes, et notamment l'absorption, la dispersion lumineuse, et analogue.

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