Interaction screening methods, systems and devices

C - Chemistry – Metallurgy – 40 – B

Patent

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Details

C40B 30/04 (2006.01) G01N 33/53 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2697294

An interaction screening method for identifying binding moieties encapsulates candidate binding moieties in droplets with a first known moiety and a second known moiety. The candidate binding moieties are different in the different droplets. The method further comprises determining for one or more of the droplets whether the candidate binding moiety is bound to the first known moiety and/or the second known moiety. Optionally, the method further comprises segregating at least one droplet in which the candidate binding moiety is bound to the first known moiety or to the first and second known moiety.

Cette invention a trait à un procédé de dépistage d'interactions permettant d'identifier des fractions de liaison, ledit procédé encapsulant les fractions de liaison candidates dans des gouttelettes avec une première fraction connue et une seconde fraction connue. Les fractions de fixation candidates sont différentes dans les différentes gouttelettes. Le procédé comprend par ailleurs la détermination pour une ou plusieurs des gouttelettes de savoir si la fraction de fixation candidate est liée à la première fraction connue et/ou à la seconde fraction connue. Éventuellement, le procédé comprend par ailleurs la séparation d'au moins une gouttelette où la fraction de fixation candidate est liée à la première fraction connue ou à la première et à la seconde fraction connue.

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