Interaction trap system for isolating novel proteins

C - Chemistry – Metallurgy – 12 – Q

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12Q 1/68 (2006.01) C07K 14/47 (2006.01) C12N 1/19 (2006.01) C12N 9/12 (2006.01) C12N 9/16 (2006.01) C12N 15/10 (2006.01) C12N 15/81 (2006.01) G01N 33/53 (2006.01) G01N 33/574 (2006.01) G01N 33/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2148252

Disclosed is a method for determining whether a first protein is capable of physically interacting with a second protein. The method involves: (a) providing a host cell which contains (i) a reporter gene operably linked to a protein binding site; (ii) a first fusion gene which expresses a first fusion protein, the first fusion protein including the first protein covalently bonded to a binding moiety which is capable of specifically binding to the protein binding site; and (iii) a second fusion gene which expresses a second fusion protein, the second fusion protein including the second protein covalently bonded to a weak gene activating moiety; and (b) measuring expression of the reporter gene as a measure of an interaction between the first and the second proteins. Such a determination facilitates the isolation of the gene encoding the interacting protein. Also disclosed herein is recombinant Cdi1 polypeptide, nucleic acid encoding the Cdi1 polypeptide, and uses thereof.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Interaction trap system for isolating novel proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Interaction trap system for isolating novel proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Interaction trap system for isolating novel proteins will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1776424

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.