C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/53 (2006.01) A01H 5/00 (2006.01) C12N 9/02 (2006.01) C12N 15/82 (2006.01)
Patent
CA 2326687
A method is provided for modifying a fatty acyl desaturase to a fatty acyl hydroxylase consisting of identifying and changing as few as four amino acid residues that are conserved in functionally equivalent desaturase enzymes from various plant species but that are not identical in fatty acyl hydroxylases that exhibit significant overall sequence similarity to the fatty acyl desaturases, and which catalyze hydroxylation at one of the carbon residues on the fatty acyl substrate that is desaturated by the corresponding desaturase; the modifications being made by changing the amino acid residue so that it is identical or functionally equivalent to the amino acid residue found in the naturally occurring hydroxylase. Also provided is a similar method of modifying a fatty acyl hydroxylase to a fatty acyl desaturase by changing seven or fewer amino acid residues. Transgenic plants and products of such transgenic plants wherein the plants have been modified to produce a modified hydroxylase or desaturase are also provided.
La présente invention concerne un procédé permettant de modifier en hydroxylase d'acyle gras une désaturase d'acyle gras. Ce procédé consiste à identifier et modifier un maximum de quatre radicaux acide aminé qui sont conservés dans des désaturases fonctionnellement équivalentes issues de diverses espèces végétales, mais qui ne sont pas identiques quant aux hydroxylases d'acyles gras présentant une importante ressemblance de séquence d'ensemble avec les désaturases d'acyles gras, et qui catalysent l'hydroxylation au niveau de l'un des radicaux carbone du substrat acyle gras qui est désaturé par la désaturase correspondante. Les modifications consistent en un changement du radical acide aminé qui est identique ou fonctionnellement équivalent au radical acide aminé se trouvant dans l'hydroxylase naturellement présente. L'invention concerne également un procédé semblable visant à modifier en désaturase d'acyle gras une hydroxylase d'acyle gras en modifiant un maximum de sept radicaux acide aminé. L'invention concerne enfin des plantes transgéniques et des produits de telles plantes transgéniques, auquel cas les plantes ont été modifiées pour produire une hydroxylase ou une désaturase modifiée.
Broun Pierre
Shanklin John
Somerville Chris
Whittle Edward J.
Brookhaven Science Associates
Carnegie Institution Of Washington
Gowling Lafleur Henderson Llp
Monsanto Company Inc.
LandOfFree
Interconversion of plant fatty acid desaturases and... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Interconversion of plant fatty acid desaturases and..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Interconversion of plant fatty acid desaturases and... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1523963