Interference with viral ires-mediated translation by a small...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) A61K 38/17 (2006.01) C07K 14/47 (2006.01) C12N 15/11 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2329155

Peptides and RNA oligonucleotides and methods of use for the inhibition of translation of an mRNA, which is initiated at an internal ribosome entry site of the mRNA and requires binding of a protein factor to that site, are disclosed. Peptides comprising the La autoantigen binding domain (LAP) are disclosed. LAP peptides alone or in combination with inhibitor RNA oligonucleotides (IRNA) may be used as antiviral agents to inhibit internal ribosome entry site (IRES) mediated viral replication.

L'invention concerne des peptides et des oligonucléotides d'ARN ainsi que leurs méthodes d'utilisation pour l'inhibition de la translation d'un ARNm, amorcée au niveau d'un site d'entrée ribosomique interne dudit ARNm et nécessitant la liaison d'un facteur protéique à ce site. Des peptides comprenant le domaine de fixation de l'autoantigène La (LAP) sont décrits. Des peptides à LAP, seuls ou combinés aux oligonucléotides d'ARNm inhibiteurs peuvent être utilisés comme agents antiviraux pour l'inhibition de la réplication virale induite par le site d'entrée ribosomique interne.

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