Interferon-.beta. complex

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/565 (2006.01) A61K 38/21 (2006.01) A61K 47/48 (2006.01)

Patent

CA 2536643

An interferon-.beta./polyethylene glycol composite which has high bioactivity; and a process for producing the composite at a high efficiency. The process for interferon-.beta. composite production is characterized by bonding interferon-.beta. to polyethylene glycol in the presence of at least one additive selected from the group consisting of oligosaccharides constituted of up to 5 sugar units, monosaccharides, sugar alcohols derived from these saccharides, and C2-6 polyhydric alcohols. The interferon-.beta. composite produced by the process has polyethylene glycol specifically bonded to the lysine located in the 19th or 134th position in the amino acid sequence of interferon-.beta..

L'invention porte sur un composite interféron .beta./polyéthylèneglycol ayant une bioactivité élevée, et sur un procédé de production de ce composite à haut rendement. Le procédé de production du composite comprenant l'interféron .beta. est caractérisé par la liaison de l'interféron .beta. au polyéthylèneglycol en présence d'au moins un additif sélectionné dans le groupe comprenant des oligosaccharides possédant jusqu'à 5 unités sucre, des monosaccharides, des alcools de sucre dérivés de ces saccharides et des polyols C¿2-6?. Le composite comprenant l'interféron .beta., produit selon ce procédé, possède un polyéthylèneglycol spécifiquement lié à la lysine située en 19ème ou 134ème position dans la séquence d'acides aminés de l'interféron .beta..

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