Interferon proteins and method of producing same

C - Chemistry – Metallurgy – 07 – K

Patent

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167/103.1, 195/1

C07K 14/56 (2006.01) A61K 39/44 (2006.01) C07K 16/24 (2006.01) C12P 21/00 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1168151

ABSTRACT OF THE DISCLOSURE Processes are provided for producing specifically recited human Le form interferon proteins. These processes comprise either subjecting a solution containing human Le form interferon proteins to affinity chroma- tography such that the human Le form interferon proteins are selectively retained, and eluting the retained interferon proteins; or cultivating a microorganism carrying DNA coding for the production of the Le form inter- feron proteins, and recovering the proteins from the culture medium. Such strictiy monospecific anti-interferon is extremely useful for antibody af- finity chromatography for purification of crude or partially purified inter- feron to obtain, in a simple and economic way, large amounts of pure Inter- feron or highly purified interferon for clinical purposes, standardization, chemical studies, sequence studies, and as immunogen for repeated preparation of monospecific anti-interferon. The monospecific anti-interferon is also useful for establishing a genetic engineering system for the production of interferon protein.

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