Internal calibration standards for electrophoretic analyses

Details Internal calibration standards for electrophoretic analyses Internal calibration standards for electrophoretic analyses

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07H 21/02 (2006.01) C12M 1/00 (2006.01) C12M 1/34 (2006.01) C12N 15/09 (2006.01) G01N 27/26 (2006.01) G01N 27/447 (2006.01) G06F 19/00 (2006.01) G01N 33/483 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2453360

The present teachings relate, among other things, to polynucleotide sequencing, fragment analysis and sample/lane tracking, and to polynucleotide sequencers and analyzers that employ optical detection techniques. Embodiments of the present teachings are described which include, for example, the addition of a calibration standard to a sequencing reaction. Information such as peak spacing and peak shape can he extracted from the standard.

La présente invention concerne, entre autres, le séquençage polynucléotidique, l'analyse de fragments et le suivi échantillon/voie, ainsi que des séquenceurs et des analyseurs polynucléotidiques utilisant des techniques de détection optique. Des modes de réalisation de la présente invention comprennent, par exemple, l'ajout d'une norme d'étalonnage à une réaction de séquençage. Cette norme permet d'extraire des informations telles que l'espacement des crêtes et la forme des crêtes.

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