Internalization

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/10 (2006.01)

Patent

CA 2672393

A target internalized within a cell (and a binding member that specifically binds thereto) can be identified in an efficient manner by segregating (or substantially segregating) genetic material encoding the binding member from genetic material encoding a binding member that binds to a target that is not internalized. This can be achieved by employing a display library of binding members having a genotype/phenotype linkage via a non-fusion protein format, whereby genetic material encoding non-internalized targets can be segregated (or substantially segregated) without lysing the cells. Internalized genetic material subsequently can be isolated and amplified.

L'invention une cible internalisée dans une cellule (et un élément de liaison qui se lie spécifiquement à cette cible), ladite cible pouvant être identifiée de manière efficace en ségrégant (ou en ségrégant sensiblement) le matériel génétique codant pour l'élément de liaison du matériel génétique codant pour un élément de liaison qui se lie à une cible qui n'est pas internalisée. Ceci est réalisé en utilisant une librairie d'éléments de liaison ayant une liaison génotype/phénotype via un format n'étant pas celui d'une protéine de fusion, le matériel génétique codant pour les cibles non internalisées pouvant ainsi être ségrégué (ou sensiblement ségrégué) sans lyser les cellules. Le matériel génétique internalisé peut ensuite être isolé et amplifié.

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