Intracellular delivery of small molecules proteins and...

A - Human Necessities – 61 – K

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A61K 38/10 (2006.01) A61K 38/14 (2006.01) A61K 38/16 (2006.01) C07H 19/00 (2006.01) C07H 21/04 (2006.01) C07K 7/08 (2006.01) C12N 15/63 (2006.01) C12P 19/34 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2516677

An amino acid sequence Arg-Lys-Met-Leu-Lys-Ser-Thr-Arg-Arg-Gln-Arg-Arg (SEQ ID NO: 1) functions as a protein transduction domain (PTD) and is capable of delivering small molecules, proteins, and nucleic acids to an intracellular compartment of a cell. An amino terminal lysine linker improves the efficiency of the PTD. A nuclear localization signal can be used to target the PTD to a cell's nucleus. The PTD can be used in PTD-cargo moiety complexes that can reversibly immortalize cells and increase cell viability in culture.

Une séquence d'acides aminés Arg-Lys-Met-Leu-Lys-Ser-Thr-Arg-Arg-Gln-Arg-Arg (SEQ ID NO: 1) fonctionne comme un domaine de transduction de protéine (PTD) et est capable de libérer des petites molécules, des protéines et des acides nucléiques dans un compartiment intracellulaire d'une cellule. Un segment de liaison lysine en N-terminal améliore l'efficacité du PTD. Un signal de localisation nucléaire peut être utilisé pour diriger le PTD sur le noyau d'une cellule. Le PTD peut être utilisé dans des complexes de fragments du type cargaison-PTD pouvant immortaliser de manière réversible des cellules et augmenter la viabilité des cellules dans la culture.

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