Intrinsic factor - horse radish peroxidase conjugates and a...

Details Intrinsic factor - horse radish peroxidase conjugates and a... Intrinsic factor - horse radish peroxidase conjugates and a...

C - Chemistry, Metallurgy

12

N

C12N 9/96 (2006.01) C12N 9/08 (2006.01) G01N 33/535 (2006.01) G01N 33/82 (2006.01)

Patent

CA 2104412

This invention presents a non-isotopic competitive assay for Vitamin B12 (B12), utilizing intrinsic factor (IF) labelled with horse radish peroxidase (HRP), by coupling via heterobifunctional cross-linking agents. In addition, a method for stabilizing the resultant conjugates by pretreatment with N-ethylmaleimide is disclosed.

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