Introduction of nutrient medium into a fermenter

C - Chemistry – Metallurgy – 12 – M

Patent

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195/143, 195/45,

C12M 1/00 (2006.01) C12M 1/08 (2006.01) C12N 1/00 (2006.01) C12N 1/32 (2006.01)

Patent

CA 1052715

ABSTRACT A method and apparatus for continuous fermentation wherein a limiting nutrient such as a carbon source is supplied to a culture at sufficient positions and at suitable rates to ensure that another nutrient such as oxygen does not effectively become the limiting nutrient. The invention is particularly applicable to the cultivation of bacteria such as Pseudomonas methylotropha upon methanol where it is undesirable that oxygen should become the limiting nutrient in any part of the culture.

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