Inverted darkfield contrast microscope and method

Details Inverted darkfield contrast microscope and method Inverted darkfield contrast microscope and method

G - Physics

02

B

G02B 21/10 (2006.01) G01N 33/487 (2006.01) G02B 21/00 (2006.01) G02B 21/02 (2006.01) G02B 21/36 (2006.01) G02B 27/00 (2006.01)

Patent

CA 2365093

A novel microscope and method of obtaining images includes a combination of the conventional darkfield illuminations technique with electronic image inversion (converting a positive to a negative image) and other improvements to further enhance the contrast and resolution of the final image. The microscope and method are referred to herein as Inverted Darkfield Contrast (IDC) and are believed to be particularly suitable for viewing live cells in real time with no staining or preparation.

Cette invention a trait à un nouveau microscope ainsi qu'à une méthode d'obtention d'images associant la technique classique d'éclairage à fond sombre à une inversion d'image électronique (conversion d'une image positive en image négative) et s'appuyant sur d'autres améliorations pour renforcer le contraste et augmenter la résolution de l'image finale. On estime que ce microscope et la méthode afférente, dénommés dans le cadre de cette invention contraste fond noir inversé (IDC), conviendront particulièrement à l'examen de cellules vivantes en temps réel et ce, sans coloration ou préparation quelconques.

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