Inverted latency specific binding assay

Details Inverted latency specific binding assay Inverted latency specific binding assay

G - Physics

01

N

167/43, 150/15.1

G01N 33/542 (2006.01) G01N 33/547 (2006.01) G01N 33/554 (2006.01)

Patent

CA 1231048

ABSTRACT A specific binding assay composition and method for determining a ligand in a sample are disclosed. The composi- tion comprises (a) a binding partner for the ligand; (b) a detection system which has at least two components; (c) a selectively accessible vesicle having a surface-incorporated ligand or ligand analog and a first component of the detection system therein; (d) a substance which modifies vesicle accessibility in response to binding of surface-associated ligand or ligand analog and binding partner; and (e) at least one additional component of the detection system which is reactive with the first component to produce a detectable response which is reduced by association of the binding partner and vesicle modifying substance with the variabily accessible vesicle. A decrease in signal is measured upon reaction as compared to the signal of unreacted vesicles.

460345

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