Ion capture reagents and methods for performing binding assays

Details Ion capture reagents and methods for performing binding assays Ion capture reagents and methods for performing binding assays

C - Chemistry, Metallurgy

08

G

530/13, 402/227,

C08G 69/48 (2006.01) C07K 17/00 (2006.01) C08G 69/10 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01) G01N 33/531 (2006.01) G01N 33/545 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1341592

This invention presents novel reagents, separation techniques and assay procedures which allow both the indicator and the capture reagents to be in solution to avoid problems of slowed immunoreaction kinetics. The separation procedure involves a soluble capture reagent, comprising a specific binding member attached to a charged substance, and an insoluble solid phase that is oppositely charged with respect to the capture reagent. A test sample suspected of containing the analyte of interest is mixed with the capture reagent to form a charged capture reagent/analyte complex. The reaction mixture is contacted to the oppositely charged solid phase to attract, attach, and separate the capture reagent/analyte complex from the reaction mixture. With an appropriate indicator reagent, i.e., a second specific binding substance which is conjugated to a label capable of producing a detectable signal, both sandwich and competitive assays can be performed.

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