G - Physics – 01 – N
Patent
G - Physics
01
N
167/44, 150/8
G01N 33/53 (2006.01) C12Q 1/26 (2006.01) C12Q 1/28 (2006.01) C12Q 1/68 (2006.01) G01N 33/58 (2006.01)
Patent
CA 1286986
Abstract of the Disclosure Useful for visualizing biological materials in a solid phase, on a gel, or in a liquid phase ls a solid salt of the meriquinone of benzidine or a substituted benzidine. An immobilized or dissolved complex of a polymeric anion and the meriquinone of benzidine or a substituted benzidine having controllable solubility may also be employed. Preferred are meriquinone salts and complexes of 3,3,5,5'-tetramethylbenzidine. For visualization, the benzidine or substituted benzidine is oxidized to its meriquinone at pH 3 to 7 in the presence of an effective anion or polymeric anion, an oxidation catalyst, and an effective amount of oxidant to form a solid salt or immobilized complex of the meriquinone under conditions where the meriquinone solubility lies below about 10-5 M.
517983
Bloch Will
Goodson Robert J.
Sheridan Patrick J.
Wada H. Garrett
Borden Ladner Gervais Llp
F. Hoffmann-La Roche Ag
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