Ip3 protein binding assay

G - Physics – 01 – N

Patent

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G01N 21/76 (2006.01)

Patent

CA 2503228

Protein binding assays are provided for determining IP3 in a sample employing as reagents a conjugate of IP3 joined at the 2-oxy through a bond or linking group to a detectable label and a truncated portion of the extracellular fragment of an IP3R. The reagents are combined with the sample and the amount of IP3 determined by means of the detectable label. The conjugate with the enzyme donor fragment of .beta.-galactosidase or a fluorescer is specifically described.

L'invention se rapporte à des biodosages par liaison de protéine qui permettent de mesurer IP¿3? dans un échantillon et qui mettent en oeuvre en tant que réactifs un conjugué de IP¿3? assemblé en 2-oxy, par l'intermédiaire d'une liaison ou d'un groupe de liaison, à une étiquette pouvant être décelée, ainsi qu'une partie tronquée du fragment extracellulaire d'un IP¿3?R. Les réactifs sont combinés à l'échantillon et la quantité de IP¿3? est mesurée au moyen de l'étiquette pouvant être détectée. L'invention se rapporte spécifiquement au conjugué doté du fragment donneur d'enzyme de la .beta.-galactosidase ou à un élément fluorescent.

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