Irreversible electroporation to create tissue scaffolds

A - Human Necessities – 61 – L

Patent

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A61L 27/36 (2006.01)

Patent

CA 2722296

The present invention provides engineered tissue scaffolds, engineered tissues, and methods of using them. The scaffolds and tissues are derived from natural tissues and are created using non-thermal irreversible electroporation (IRE). Use of IRE allows for ablation of cells of the tissue to be treated, but allows vascular and neural structures to remain essentially unharmed. Use of IRE thus permits preparation of thick tissue scaffolds and tissues due to the presence of vasculature within the scaffolds. The engineered tissues can be used in methods of treating subjects, such as those in need of tissue replacement or augmentation.

La présente invention concerne des échafaudages tissulaires modifiés, des tissus modifiés et des procédés dutilisation de ceux-ci. Les échafaudages et les tissus sont dérivés de tissus naturels et sont créés au moyen de lélectroporation irréversible non-thermique (IRE). Lutilisation de lIRE permet lablation de cellules du tissu devant être traité, tout en laissant les structures vasculaires et neurales essentiellement indemnes. Lutilisation de lIRE permet ainsi la préparation de tissus et déchafaudages tissulaires épais grâce à la présence dun réseau vasculaire dans les échafaudages. Les tissus modifiés peuvent être utilisés dans des procédés de traitement de sujets, tels que ceux ayant besoin dun remplacement ou dune augmentation tissulaire.

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