C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 11/08 (2006.01) C08G 18/00 (2006.01) C12N 11/04 (2006.01)
Patent
CA 2454826
This invention provides a method of irreversibly immobilizing an enzyme into polyurethane coatings. This invention also provides for an enzyme-containing coating having a degree of immobilization of the enzyme of approximately 100%. The synthesis of waterborne polyurethane coatings in the presence of enzyme has enabled the irreversible attachment of the enzyme to the polymeric matrix. The distribution of immobilized enzyme as well as activity retention are homogeneous within the coating. Decreasing ECC hydrophobicity, via the use of a less hydrophobic polyisocyanate prepolymer during polymerization, significantly enhanced the intrinsic activity of the ECC.
La présente invention concerne un procédé permettant l'immobilisation irréversible d'enzymes dans des revêtements de polyuréthane. L'invention concerne également un revêtement contenant des enzymes et présentant un taux d'immobilisation des enzymes approchant les 100 %. En l'occurrence, la synthèse de revêtement de polyuréthane à l'eau a permis la fixation irréversible de l'enzyme à la matrice polymère. La distribution d'enzyme immobilisé ainsi que le maintien d'activité restent homogènes dans le revêtement. La décroissance de l'hydrophobicité des revêtements contenant des enzymes résultant de l'utilisation d'un prépolymère de polyisocyanate moins hydrophobe pendant la polymérisation a renforcé de façon remarquable l'activité intrinsèque des revêtements contenant des enzymes.
Drevon Geraldine F.
Russell Alan J.
Borden Ladner Gervais Llp
University Of Pittsburgh
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