Irreversible immobilization of enzymes into polyurethane...

C - Chemistry – Metallurgy – 12 – N

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C12N 11/08 (2006.01) C08G 18/00 (2006.01)

Patent

CA 2567359

A method of irreversibly immobilizing an enzyme in a polyurethane and an enzyme-containing polyurethane having a degree of immobilization of the enzyme of approximately 100%. The synthesis of waterborne polyurethanes in the presence of enzyme has enabled the irreversible attachment of the enzyme to the polymeric matrix. The distribution of immobilized enzyme as well as activity retention are homogeneous within the polyurethane. Decreasing ECC hydrophobicity, via the use of a less hydrophobic polyisocyanate prepolymer during polymerization, significantly enhanced the intrinsic activity of the ECC.

La présente invention concerne, d'une part un procédé permettant d'immobiliser irréversiblement une enzyme dans un polyuréthane, et d'autre part un polyuréthane présentant un certain degré d'immobilisation de l'enzyme d'approximativement 100 %. La synthèse de polyuréthane à l'eau en présence d'enzyme a permis la liaison irréversible de l'enzyme à la matrice polymère. A l'intérieur du polyuréthane, la distribution de l'enzyme immobilisée ainsi que la rétention d'activité sont homogènes. La moindre hydrophobicité des revêtements contenant des enzymes a notablement renforcé l'activité intrinsèque de ces revêtement, du fait de l'utilisation de prépolymères de polyisocyanates moins hydrophobes pendant la polymérisation.

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