Isoamylase gene from flaviobacterium sp., compositions...

Details Isoamylase gene from flaviobacterium sp., compositions... Isoamylase gene from flaviobacterium sp., compositions...

C - Chemistry, Metallurgy

12

N

C12N 15/56 (2006.01) A01H 5/00 (2006.01) C08B 30/00 (2006.01) C12N 9/12 (2006.01) C12N 9/44 (2006.01) C12N 15/54 (2006.01) C12N 15/82 (2006.01) C12P 19/16 (2006.01) C12S 3/12 (2006.01)

Patent

CA 2195786

A method of producing plant products containing modified starch content, including higher ratios of amylose to amylopectin, increase in intermediate material, or amylopectin having fewer branches or altered branching pattern. Also provided are DNA constructs and transformed plant cells useful in that method. The preferred method uses isoamylase from a Flavobacteriumsp., more preferrably in combination with a gene encoding ADPglucose pyrophosphorylase. Also disclosed are the gene from Flavobacteriumsp. and transformed bacterial and plant cells containing a derivative thereof.

Procédé de production de produits à base de végétaux contenant de l'amidon modifié, se caractérisant par un rapport amylose/amylopectine plus élevé, un accroissement en produits intermédiaires ou une amylopectine ayant moins de ramifications ou à ramifications modifiées. L'invention porte également sur des ADN de recombinaison et des cellules végétales modifiées obtenus par ce procédé. Selon le procédé préféré, on emploie de l'isoamylase provenant d'un Flavobacterium sp. de préférence combinée à un gène codant pour l'ADP-glucose pyrophosphororylase. Sont également présentés le gène du Flavobacterium sp. et des cellules transformées de bactéries et de plantes contenant l'un de ses dérivés.

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