G - Physics – 01 – N
Patent
G - Physics
01
N
204/99.02, 204/1
G01N 27/26 (2006.01) G01N 27/447 (2006.01)
Patent
CA 1335805
Described are an isoelectric focusing electrophoretic process and an apparatus for the separation and purification of an amphoteric or neutral chemical compound from one or more electrically charged chemical com- pound(s), e.g. contaminating proteins and salts. The apparatus comprises a flow chamber (8) which is connected via a cylinder (5) to the cathodic chamber (3) and via another cylinder (12) to the anodic chamber (14). The mixture to be separated is present within a hydraulic flow in the flow chamber (8). Cylinders (5) and (12) contain immobilized pH-gradients or amphoteric isoelectric pH-membranes. Each of said pH-gradients and pH-membranes has conductivity and both buffering and titrant capacity in its pH-interval. The extremities of said gradients or pH-membranes forming the ceiling and the floor of said flow chamber (8) have iso- electric points equal to or just higher and just lower than the iso- electric point of said amphoteric compound of interest which is kept at its isoelectric point in the hydraulic flow and does not enter said pH-gradients and pH-membranes. Contrary thereto the electrically charged chemical compounds, e.g. the contaminating proteins and salts, are driven by an electric field into said pH-gradients or via said pH-gradients or pH-membranes into the electrolyte reservoirs (3) and (14). In respect to the prior art, the described process has the advantage that the desired compound need not be detected and extracted from any matrix, e.g. from said pH-gradients, and that the recovery and purity of the desired compound is higher.
563616
Faupel Daniel Michel
Righetti Pier Giorgio
Fetherstonhaugh & Co.
Novartis Ag
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