Isolated alcohol dehydrogenase enzymes and uses thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/53 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 9/04 (2006.01) C12P 7/58 (2006.01) C12P 19/02 (2006.01)

Patent

CA 2712722

Bacterial polynucleotides and polypeptides are provided in which the polypeptides have a dehydrogenase activity, such as an alcohol dehydrogenase (ADH) activity, an uronate, a 4-deoxy-L- erythro-5-hexoseulose uronate (DEHU) ((4S,5S)-4,5 dihydroxy-2,6-dioxohexanoate) hydrogenase activity, a 2-keto-3-deoxy-D- gluconate dehydrogenase activity, a D-mannuronate hy-drogenase activity, and/or a D-mannnonate dehydrogenase activity. Methods, enzymes, recombinant microorganism, and micro-bial systems are also provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided.

L'invention se rapporte à des polynucléotides et à des polypeptides bactériens parmi lesquels les polypeptides présentent une activité déshydrogénase comme l'activité alcool déshydrogénase (ADH), un uronate, une activité 4-désoxy-L-érythro-5-hexoseulose uronate (DEHU) ((4S,5S)-4,5 dihydroxy-2,6-dioxohexanoate) hydrogénase, une activité 2-céto-3-désoxy-D-gluconate déshydrogénase, une activité D-mannuronate hydrogénase et/ou une activité D-mannonate déshydrogénase. Des procédés, enzymes, microorganismes recombinants et systèmes microbiens sont également proposés pour transformer des polysaccharides tels que ceux issus de la biomasse en monosaccharides ou oligosaccharides adéquats ainsi que pour transformer les monosaccharides ou oligosaccharides adéquats en produits chimiques de base comme les biocarburants. Les produits chimiques de base produits par les procédés décrits dans la présente sont également proposés.

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