Isolated and purified nucleic acids comprising a gene...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/54 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2321180

An isolated and purified nucleic acid comprising a gene specifically expressed in hop lupulin glands. Hops are dioecious, and only female plants bear cones, the lupulin glands of which contain secondary metabolic product which provide bitterness and flavor to beer. These secondary metabolic products contain some pharmacologically effective compounds. In order to breed a more useful cultivar of hops by manipulating the constituent of such useful secondary metabolic products relying on genetic engineering techniques, this invention provides an isolated and purified nucleic acid comprising a gene specifically expressed in hop lupulin glands. By using this nucleic acid, it is possible to develop a novel method for breeding hops with transformation techniques and molecular selection techniques. Furthermore, the present invention also provides a nucleic acid comprising the regulatory region for sepcifically expressing genes in lupulin glands. This nucleic acid can be used also for hop breeding.

L'invention concerne un acide nucléique isolé et purifié comprenant un gène exprimé spécifiquement dans les glandes à lupuline du houblon. Le houblon est une plante dioecique exclusivement femelle à cônes, dont les glandes à lupuline renferment des produits métaboliques secondaires qui confèrent à la bière sont goût amer et son parfum. Lesdits produits secondaires renferment à leur tour des composés ayant une efficacité pharmacologique. De manière à pouvoir cultiver une variété plus utile de houblon en manipulant le contenu efficace desdits produits par le biais du génie génétique, on obtient un acide nucléique isolé et purifié renfermant un gène exprimé spécifiquement dans les glandes à lupuline du houblon. L'acide nucléique en question permet de mettre au point un nouveau procédé de culture du houblon, via des techniques de transformation et de sélection moléculaire. L'invention concerne également un acide nucléique comprenant la région de régulation correspondant à des gènes exprimés spécifiquement dans les glandes considérées. On peut également utiliser cet acide nucléique pour la culture du houblon.

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