Isolated dna encoding human h3 histamine receptor

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) A61K 31/00 (2006.01) A61K 31/785 (2006.01) A61P 25/00 (2006.01) C07K 14/705 (2006.01) C07K 14/72 (2006.01) C07K 16/28 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01) G01N 33/566 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2312274

DNAs encoding the human histamine H3 receptor have been cloned and characterized. The recombinant protein is capable of forming biologically active histamine H3 receptor protein. The cDNAs have been expressed in recombinant host cells which produce active recombinant protein. The recombinant protein is also purified from the recombinant host cells. In addition, the recombinant host cells are utilized to establish a method for identifying modulators of the receptor activity, and receptor modulators are identified.

Des ADN codant pour le récepteur H3 d'histamine humain ont été clonés et caractérisés. La protéine de recombinaison est capable de former une protéine récepteur H3 d'histamine biologiquement active. Les ADNc ont été exprimés dans des cellules hôtes de recombinaison qui produisent la protéine de recombinaison active. Ladite protéine de recombinaison est également purifiée à partir des cellules hôtes de recombinaison. De plus, les cellules hôtes de recombinaison sont utilisées pour établir une méthode d'identification de modulateurs de l'activité de récepteur, et des modulateurs de récepteur ont ainsi été identifiés.

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