Isolated nucleic acid molecules useful as leukemia markers...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/12 (2006.01) C07K 14/47 (2006.01) C07K 14/82 (2006.01) C07K 16/18 (2006.01) C07K 16/30 (2006.01) C12Q 1/68 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2228999

The present invention relates to four novel human genes amplified and overexpressed in breast carcinoma and located on the q11-q21.3 region of chromosome 17. The four novel genes are useful in breast cancer prognosis. The present invention also relates to a fifth novel human gene expressed in breast carcinoma and located on chromosone 6q22-q23. A sixth novel gene is also described that is the murine homolog of the human D52 gene. The genes and gene fragments of the present invention are themselves useful as DNA and RNA probes for gene mapping by in situ hybridization with chromosomes and for detecting gene expression in human tissues (including breast and lymph node tissues) by Northern blot analysis.

La présente invention concerne quatre nouveaux gènes humains amplifiés et surexprimés dans le cancer du sein. Ces gènes se trouvent dans la région q11-q21.3 du chromosome 17. Les quatre nouveaux gènes sont utiles pour pronostiquer un cancer du sein. La présente invention concerne également un cinquième nouveau gène humain exprimé dans le cancer du sein et situé sur le chromosome 6q22-q23. Elle concerne également un sixième nouveau gène qui est l'homologue murin du gène humain D52. Les gènes et les fragments de gènes de la présente invention sont utiles pour préparer des sondes d'ADN ou d'ARN pour la cartographie de gènes par l'hybridation in situ avec des chromosomes et pour la détection de l'expression de gènes dans les tissus humains (en particulier les tissus des seins et des ganglions lymphatiques) par la méthode Northern blot.

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