Isolated spinach rubisco large subunit n-methyltransferase

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/54 (2006.01) A01H 5/00 (2006.01) C12N 9/10 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2261775

The gene sequence for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit (LS) .epsilon.N-methyltransferase (protein methylase III or Rubisco LSMT) from a plant which has a des(methyl) lysyl residue in the LS is disclosed. In addition, the full-length cDNA clones for Rubisco LSMT are disclosed. Transgenic plants and methods of producing same which have the Rubisco LSMT gene inserted into the DNA are also provided. Further, methods of inactivating the enzymatic activity of Rubisco LSMT are also disclosed.

Séquence génique de ?.epsilon.¿N-méthyltransférase (méthylase III protéique ou LSMT de rubisco) de sous-unités importantes (LS) de ribulose-1,5-bisphosphate carboxylase/oxygénase (rubisco) d'une plante qui possède un reste des(méthyl) lysyle dans la sous-unité importante. La présente invention concerne en outre les clones d'ADNc pleine longueur de LSMT de rubisco, des plantes transgéniques et des procédés de production desdites plantes dans l'ADN desquelles le gène LSMT de rubisco a été introduit, ainsi que des procédés permettant d'inactiver l'activité enzymatique de LSMT de rubisco.

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