Isolating a cytoplasmic fraction without impairing the...

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G01N 33/48 (2006.01) C12Q 1/68 (2006.01) G01N 33/487 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2362486

A method of isolating a cytoplasmic fraction from an oocyte which does not impair the capacity of the oocyte to be fertilized, the method including the step of releasing a cytoplasmic fraction from the oocyte which is about 5 % of the volume of the oocyte. A method of isolating a cytoplasmic fraction from an embryonic cell which does not impair the developmental potential of the cell, the method including the step of releasing a cytoplasmic fraction from the cell which is about 5 % of the volume of the cell.

La présente invention concerne une méthode d'isolement d'une fraction cytoplasmique d'un ovocyte, n'altèrant pas la capacité de l'ovocyte à être fertilisé, et consistant à séparer une fraction cytoplasmique de l'ovocyte correspondant à environ 5 % du volume de l'ovocyte. L'invention concerne également une méthode d'isolement d'une fraction cytoplasmique d'une cellule embryonnaire, n'altérant pas le potentiel de développement de la cellule, ladite méthode consistant à séparer une fraction cytoplasmique de la cellule correspondant à environ 5 % du volume de la cellule.

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