Isolating biological modulators from biodiverse gene...

C - Chemistry – Metallurgy – 12 – N

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Details

C12N 15/11 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01) C40B 40/02 (2006.01) C40B 40/08 (2006.01) C40B 50/06 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2372464

The present invention provides a method for identifying a modulator or mediator of a biological activity, which activity includes antigenicity and/or immunogenicity, said method comprising the steps of: (i) producing a gene fragment expression library derived from defined nucleotide sequence fragments; and (ii) assaying the expression library for at least an amino acid sequence derived from step (i) for a biological activity wherein that activity is different from any activity the amino acid sequence may have in its native environment.

La présente invention concerne un procédé permettant d'identifier un modulateur ou un médiateur d'activité biologique, laquelle activité comprend le pouvoir antigénique et/ou l'antigénicité. Ce procédé consiste (i) à produire une banque d'expression de fragments de gènes dérivée de fragments de séquences nucléotidiques déterminés; et (ii) à analyser la banque d'expression d'au moins une séquence d'acides aminés dérivée de l'étape (i) à la recherche d'une activité biologique, cette activité biologique étant différente de toute activité que la séquence d'acides aminés peut avoir dans son environnement natif.

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